Open qPCR Machine: Your Personal Real-Time PCR Machine

Precise, Consistent qPCR Results

Ultra-tight replicates. Results so repeatable they can be compared across runs. Quantification so accurate that two-fold differences in gene expression can be detected.

Explore Performance

Powerful, User-Friendly Software

Rapidly design, run, and analyze advanced experiments.

Browser-based software is always up to date. There’s nothing to install: Open qPCR’s software runs right in your web browser on any Mac, PC, or tablet.

Explore Software

Compact, Portable, Connected

Start a run at the lab, view results from your home, or anywhere you have Internet.

Or take Open qPCR into the field. Built like a tiny tank and weighing only 4 kg, it’s easy to transport, and can run anywhere you have power.

20 cm

25 cm

30 cm

Insanely Fast

Get rapid results for rapid decision making. Open qPCR can run 40 cycles of PCR in 20 minutes, making it the device of choice for time-sensitive applications.

Fast Real-Time PCR Machine does 40 cycles in 20 minutes

Open Platform

Avoid vendor lock-in. Open qPCR works with all PCR master mixes, probe chemistries, and dyes.

Open Source

We promote research & support the community by making our core bioinformatics algorithms open source.

Open API

Control Open qPCR via your laboratory automation system or custom software with our open API.

Tech Specs

PCR

16 wells, 100 µL low profile tubes

TaqMan, Molecular Beacon, Scorpions, Hydrolysis

Chai Green, SYBR Green

FAM, HEX, VIC, JOE

10 – 50 µL

1 copy

10¹⁰

Optical

454 – 487 nm

513 – 555 nm

508 – 532, 573 – 597 nm

4.4 s

Solid-State LEDs & Photodiodes

Thermal

4 – 100 °C

0.00001 – 5.0 °C/s

±0.2 °C at 65 °C

±0.4 °C at 65 °C

Ambient – 120 °C

Connectivity

Ethernet, 802.11n WiFi, USB

Web Application

Mac, PC, Linux, iPad, Android Tablet

REST API

System

100 – 240 V, 50 – 60 Hz

10 – 35 °C

20 – 80%, non-condensing

28 x 24 x 19 cm (11 x 9.5 x 7.5 inches)

4 kg (9 lbs)

FCC, CE

Research Citations

Rudko SP, Reimink RL, Peter B, White J. (2020). Democratizing water monitoring: Implementation of a community-based qPCR monitoring program for recreational water hazards. PLoS ONE, 15(5).

Aufdembrink LM, Hoog TG, Pawlak MR, Bachan BF, Heili JM, Engelhart AE. (2019). Methods for thermal denaturation studies of nucleic acids in complex with fluorogenic dyes. (AE Hargrove, Ed.), Methods in Enzymology. (Volume 623, pp. 23-43). Cambridge (MA): Elsevier.

Damse TR, Allen PB. (2019). Idiosyncrasies of thermofluorimetric aptamer binding assays. BioTechniques, 66(5).

Damase TR, Miura TA, Parent CE, Allen PB. (2018). Application of the Open qPCR Instrument for the in Vitro Selection of DNA Aptamers against Epidermal Growth Factor Receptor and Drosophila C Virus. ACS Comb Sci., 20(2):45-54.

Taylor BJ, Lanke K, Banman SL, Morlais I, Morin MJ, Bousema T, Rijpma SR, Yanow SK. (2017). A Direct from Blood Reverse Transcriptase Polymerase Chain Reaction Assay for Monitoring Falciparum Malaria Parasite Transmission in Elimination Settings. Am J Trop Med Hyg., 97(2):533-543.