Chai Enzymatic DNA/RNA Extraction Buffer

Highlights

• Direct Extraction: Get PCR-ready DNA and RNA in two simple steps
• Low Cost: Typically $0.30 – $0.80 USD per extraction
• Easily Scalable: Extract hundreds of samples in less than half an hour
• Widely Compatible: Works for large range of sample matrices

Description

Complicated, expensive, and time-consuming nucleic acid extractions are often the headache of a lab - but they don’t have to be. Replace your current extraction kit with a single buffer that extracts PCR-ready DNA and RNA with two simple steps in 20 minutes—including mere seconds of hands-on time.

From Dollars to Cents per Extraction

Cut down cost by processing your samples with direct extraction instead of complicated, costly spin column purification and magnetic bead extraction kits. Processing samples with the Enzymatic DNA/RNA Extraction Buffer can reduce your cost per extraction to $0.30 – $0.80.

A Fast, Foolproof Workflow

Stop spending hours in the lab with tedious protocols that have you repeatedly alternating between centrifuging, pipetting, and incubating samples. With a simple two-step extraction process, hundreds of samples can be extracted simultaneously to expedite yield of DNA and RNA that is ready for PCR in less than 30 minutes.

Lysate from direct extraction can be used for a wide range of applications, including but not limited to: genotyping, gene expression, screening, bacterial detection, and viral DNA/RNA detection.

Easy Protocol

Add the buffer to your sample to extract your sample at room temperature, then briefly heat to inactivate the enzyme and use the lysate for PCR.

Maximize Recovery

Spin column purifications can concentrate samples, but nucleic acid is lost at each purification step. Enzymatic DNA/RNA Extraction Buffer avoids this with a direct extraction method.

The buffer utilizes both chemical and enzymatic mechanisms to extract samples immediately at room temperature. Chemical lysis disrupts lipid bilayers and additional buffer properties work to stabilize DNA and RNA, while enzymes digest membrane proteins and degrade DNases and RNases to maximize nucleic acid recovery.

Available at 1X and 10X

There are two concentrations available: The standard 1X concentration is ideal for solid samples such as swabs, cell pellets, tissue, and membrane filters. The 10X concentration is best for liquid samples such as water, biological fluids, or transport media. This avoids unnecessary sample dilution, increasing sensitivity.

Safe and Environmentally Conscious

Other organic lysis methods such as phenol chloroform extraction are hazardous and time-consuming; involving multiple precipitation, washing, and spinning steps. Unlike TRIzol, the Enzymatic DNA/RNA Extraction Buffer is non-toxic and safe to use outside of a fume hood. Sample extraction is performed in a single 1.5 mL tube, saving time and eliminating consumables and supplemental reagents commonly found in alternate extraction methods that will end up in landfill.

Specifications

FAQs

• Is this product suitable for clinical laboratory diagnostics?

  Yes. The 10X Enzymatic DNA/RNA Extraction Buffer has been validated for SARS-CoV-2 detection from nasopharyngeal swabs in VTM media and saliva samples, while the 1X buffer has been validated for recovery from dry swabs. The buffer was submitted as a component of Chai’s FDA EUA for the COVID-19 Saliva Dx Test Kit during the COVID-19 pandemic.

  High complexity CLIA laboratories continue to use this product in other laboratory developed tests (LDTs) to cut down on costs and time for extractions for other viral and bacterial detection assays.

• How does this buffer work?

  The buffer is unique due to its combined chemical and enzymatic lysis mechanisms that occur at room temperature. Chemical lysis occurs to initially disrupt lipid bilayers of cell membranes. Stabilizing agents within the buffer scavenge divalent ions and work to protect released DNA and RNA from degradation. Additionally, digestive enzymes degrade membrane proteins.

  The digestive enzymes further protect DNA and RNA from degradation by digesting any DNases or RNases in the sample matrix. As cells lyse, DNases and RNases are immediately degraded to maximize nucleic acid recovery.

• What sample matrices have people used this buffer with?

  The Enzymatic DNA/RNA Extraction Buffer has been validated internally or externally tested with the following matrices:

  Human: Blood, dry buccal/nasal swabs, oral fluid, saliva, swabs in viral transport media/universal transport media, urine

  Animal: Bird feathers and blood, cell cultures, cheese, chicken gizzards, mouse ear and tail, raw milk, shark fin, snake saliva, tick blood

  Bacteria: Cell cultures, cell pellets, environmental water, probiotics

  Don’t see your matrix listed? Get in touch with an application specialist to see whether our buffer would be a good fit for your sample extraction.

• Does this work for both RNA and DNA?

  The Enzymatic DNA/RNA Extraction Buffer was originally developed for sensitively detecting RNA from complex human biological matrices. The same protocol will work for both DNA extraction and RNA extraction. The enzymatic properties of the buffer degrade both DNases and RNases to allow for highly sensitive RNA detection by PCR.

• Will the buffer cause PCR inhibition?

  The buffer is not inhibitory to PCR, so high volumes of lysate may be used in PCR reactions without further purification of nucleic acid. We recommend using sample lysate in combination with an inhibition-resistant PCR master mix for the most sensitive detection.

• Is this product available for OEM?

  Chai’s Enzymatic DNA/RNA Extraction Buffer is license-free and available for OEM use. It may be used freely for any application, including commercial and diagnostic use. No license agreements or royalty payments are required for OEM use.

Resources

For Research Use Only. Not for use in diagnostic procedures.